SAIP2016

Development of non-linear microscopy infrastructure

6 Jul 2016, 16:10

1h 50m

Kramer Law building

Board: C.474

Poster Presentation Track C - Photonics Poster Session (2)

Speakers

Ms Anneke Erasmus (Laser Research Institue) Mr George Dwapanyin (Laser Research Institue) Mr Ruan Viljoen (Stellenbosch University)

Level for award<br>&nbsp;(Hons, MSc, <br> &nbsp; PhD, N/A)?

MSc

Main supervisor (name and email)<br>and his / her institution

Erich Rohwer - egr@sun.ac.za - Laser Research Institue

Please indicate whether<br>this abstract may be<br>published online<br>(Yes / No)

Yes

Would you like to <br> submit a short paper <br> for the Conference <br> Proceedings (Yes / No)?

No

Abstract content <br> &nbsp; (Max 300 words)<br><a href="http://events.saip.org.za/getFile.py/access?resId=0&materialId=0&confId=34" target="_blank">Formatting &<br>Special chars</a>

The components of a versatile multimodal non-linear microscopy setup have been developed and an integrated construction has been designed. The various critical components in this setup are discussed and elements are characterised. These include for the excitation: A coherent super continuum light source generated in a photonic crystal fibre pumped by a femtosecond laser, a 4f pulse shaper with programmable spatial light modulator for dynamic pulse compression of the super continuum and pulse manipulation, the Multiphoton Intra-pulse Interference Phase Scan (MIIPS) compression algorithm; For sample management an optical tweezer and basic imaging facility; For imaging a fluorescence detection and confocal setup with scanning facility.
Specific detail of the high intensity probe created through compression of the super continuum through phase correction using a spatial light modulator is presented. The characteristics of the pulses regarding spectral bandwidth and polarisation dependence on input pulse power, the phase and amplitude of the compressed pulses are presented. The MIIPS algorithm is described and evaluated. The details of the tweezer setup are presented and results from the instruments are discussed. A fluorescence microscope has been integrated into the system and the layout and functioning of this setup is also discussed.
The spectral imaging facility is presented and future plans for further development of the setup are alluded to.

Apply to be<br> considered for a student <br> &nbsp; award (Yes / No)?

Yes