4-8 July 2016
Kramer Law building
Africa/Johannesburg timezone
<a href="http://events.saip.org.za/internalPage.py?pageId=10&confId=86">The Proceedings of SAIP2016</a> published on 24 December 2017

The effect of low level laser therapy on both HIV infected and uninfected TZM-bl cells

6 Jul 2016, 16:10
1h 50m
Kramer Law building

Kramer Law building

UCT Middle Campus Cape Town
Board: C.283
Poster Presentation Track C - Photonics Poster Session (2)

Speaker

Ms Masixole Lugongolo (Council of Scientific and Industrial Research)

Would you like to <br> submit a short paper <br> for the Conference <br> Proceedings (Yes / No)?

No

Main supervisor (name and email)<br>and his / her institution

Dr Patience Mthunzi
pmthunzi@csir.co.za
Council of Scientific and Industrial Research (CSIR), National Laser Centre

Please indicate whether<br>this abstract may be<br>published online<br>(Yes / No)

No

Apply to be<br> considered for a student <br> &nbsp; award (Yes / No)?

Yes

Level for award<br>&nbsp;(Hons, MSc, <br> &nbsp; PhD, N/A)?

PhD

Abstract content <br> &nbsp; (Max 300 words)<br><a href="http://events.saip.org.za/getFile.py/access?resId=0&materialId=0&confId=34" target="_blank">Formatting &<br>Special chars</a>

Human immunodeficiency virus (HIV) infection still remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine or a treatment that would completely eradicate the virus from the system of the infected individuals, the quest for new therapies continues. Low level laser therapy (LLLT) involves the exposure of cells or tissues to low levels of red and near infrared light. LLLT has been widely used in different medical conditions including skin diseases, diabetes and wound healing, but not in HIV infection. This study aimed to determine the effects of LLLT on HIV uninfected and infected TZM-bl cells. Both HIV infected and uninfected TZM-bl cells were laser irradiated at a wavelength of 660 nm with different fluencies of 0, 2, 4, 6, 8 and 10 J/cm2. Changes in cellular responses were assessed using cell morphology, viability, proliferation, cytotoxicity and luciferase activity. The non-irradiated cells (0 J/cm2) and HIV uninfected cells were used as controls. TZM-bl cells irradiated in the absence of HIV showed no changes in cell morphology, viability, proliferation and cytotoxicity. However, cells irradiated in the presence of HIV infection showed changes in cell morphology, viability, proliferation and cytotoxicity. Laser irradiation reduced luciferase activity in HIV uninfected and infected cells. Laser irradiation in the absence of HIV has no inhibitory effect on cells, while in the presence of HIV infection it induces cell damage in a dose dependent manner.

Primary author

Ms Masixole Lugongolo (Council of Scientific and Industrial Research)

Co-authors

Dr Patience Mthunzi (CSIR - NLC) Dr Saturnin OMBINDA-LEMBOUMBA (CSIR- National Laser Centre) Dr Sello Manoto (CSIR)

Presentation Materials

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