4-8 July 2016
Kramer Law building
Africa/Johannesburg timezone
<a href="http://events.saip.org.za/internalPage.py?pageId=10&confId=86">The Proceedings of SAIP2016</a> published on 24 December 2017

Integrated optical tweezer and fluorescence microscope.

6 Jul 2016, 10:20
20m
5C (Kramer Law building)

5C

Kramer Law building

UCT Middle Campus Cape Town
Oral Presentation Track C - Photonics Photonics

Speaker

Ms Anneke Erasmus (Stellenbosch University)

Please indicate whether<br>this abstract may be<br>published online<br>(Yes / No)

Yes

Apply to be<br> considered for a student <br> &nbsp; award (Yes / No)?

Yes

Abstract content <br> &nbsp; (Max 300 words)<br><a href="http://events.saip.org.za/getFile.py/access?resId=0&materialId=0&confId=34" target="_blank">Formatting &<br>Special chars</a>

An integrated optical tweezer and fluorescence microscopy setup has been developed. The system allows a sample to be optically trapped using the tweezer setup and investigated further by looking at a fluorescence signal detected subsequent to an excitation laser. Optical tweezing is made possible by tightly focusing laser light onto a dielectric bead. In the focus, the difference in refractive index of the bead with respect to its surrounding environment causes a change in momentum of the focused light, which in turns imparts a force on the particle towards the centre of the focus. By attaching a fluorophore to an individual bead, the position of the fluorophore can be manipulated. The excitation laser that stimulates fluorescence in the sample (fluorophore) is coupled parallel to the optical tweezer system. Detection of this fluorescence signal at various positions on the sample permits the sample to be imaged. In this presentation the layout of the optical tweezer system and its characterization will be discussed. Piezo controllers allow x- and y- positioning of the sample. Detection using a spectrometer to record a spectrum at each x- and y- position hence allows spectral imaging of the sample. First fluorescence microscopy results will be presented. The proposed modification and adaptation of this setup to enable nonlinear microscopy techniques will be discussed. This includes combining the microscope setup with a compressed super continuum excitation source for near diffraction limited nonlinear microscopy. The nonlinear microscopy techniques to be considered is multiphoton absorption, second harmonic generation, third harmonic generation and coherent anti-Stokes Raman spectroscopy.

Main supervisor (name and email)<br>and his / her institution

(Prof. E.G. Rohwer; egr@sun.ac.za) Stellenbosch University

Would you like to <br> submit a short paper <br> for the Conference <br> Proceedings (Yes / No)?

No

Level for award<br>&nbsp;(Hons, MSc, <br> &nbsp; PhD, N/A)?

MSc

Primary author

Ms Anneke Erasmus (Stellenbosch University)

Co-authors

Prof. Erich Rohwer (University of Stellenbosch) Dr Gurthwin Bosman (Stellenbosch University) Dr Pieter Neethling (Laser Research Institute, University of Stellenbosch) Mr Ruan Viljoen (Stellenbosch University)

Presentation Materials

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