Speaker
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Main supervisor (name and email)<br>and his / her institution
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Would you like to <br> submit a short paper <br> for the Conference <br> Proceedings (Yes / No)?
No
Abstract content <br> (Max 300 words)<br><a href="http://events.saip.org.za/getFile.py/access?resId=0&materialId=0&confId=34" target="_blank">Formatting &<br>Special chars</a>
The NF-κB pathway has critical roles in cancer, immunity and inflammatory responses. Understanding the mechanism(s) by which mutations in genes involved in the pathway cause disease has provided valuable insight into its regulation, yet, many aspects remain unexplained. Several lines of evidence have led to the hypothesis that the regulatory/sensor protein NEMO acts as a biological binary switch. This hypothesis depends on the formation of a higher-order structure, which has yet to be identified, using traditional molecular techniques. Here, we use super-resolution microscopy to reveal the existence of higher-order NEMO lattice structures dependent on the presence of polyubiquitin chains prior to NF-κB activation. Such structures may permit proximity-based trans-autophosphorylation leading to cooperative activation of the signalling cascade. We further show that NF-κB activation results in modification of these structures. Finally we demonstrate that these structures are abrogated in cells derived from incontinentia pigmenti patients
Please indicate whether<br>this abstract may be<br>published online<br>(Yes / No)
Yes
Apply to be<br> considered for a student <br> award (Yes / No)?
No
