9-13 July 2012
Africa/Johannesburg timezone
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Response of low intensity laser irradiation on collagen production in diabetic wounded fibroblast cells in vitro

10 Jul 2012, 11:20
Oral Presentation Track C - Photonics Photonics


Ms Sandra Ayuk (Laser Research Center)

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Abstract content <br> &nbsp; (Max 300 words)

Ayuk, S. M., Houreld N. N. and Abrahamse H.
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg, 2028, South Africa Tel: +27 11 559-6406 Fax +27 11 559-6884 Email: habrahamse@uj.ac.za

Background: Collagen Type Ι (Col-Ι) is a major component of the extracellular matrix (ECM) and is important in wound healing processes. Several studies have shown that Low Intensity Laser Irradiation (LILI) biostimulates Col-Ι synthesis both in vitro and in vivo. Diabetic patients are known to suffer from slow-to-heal wounds and collagen production in these patients is impaired. This study aimed to determine if LILI affects collagen production and related cellular responses in an in vitro diabetic wounded fibroblast model. Method: This study was performed on isolated human skin fibroblasts. Different cell models namely; normal and diabetic wounded were used. Cells were irradiated with 5 J/cm2 at a wavelength of 660 nm and incubated for 48 or 72 hours. Non-irradiated cells (0 J/cm2) were used as controls. Cellular viability (Trypan blue), morphology (Bright Field Microscopy), proliferation (VisionBlue Quick Cell Proliferation Assay), and Col-Ι (Enzyme Linked Immunoabsorbent Assay, ELISA, and Sircol Assay) were assessed. Results: Diabetic wounded cells irradiated with 5 J/cm2 at 660 nm showed a significant increase in cell migration, viability, proliferation and Col-Ι. Conclusion: This study shows that LILI stimulates Col-Ι synthesis in diabetic wound healing in vitro at 660 nm.

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Main supervisor (name and email)<br>and his / her institution

Prof Heidi Abrahamse, habrahamse@uj.ac.za, Laser Research Center,John Orr Building, Rm 2116,University of Johannesburg.

Primary author

Ms Sandra Ayuk (Laser Research Center)


Prof. Heidi Abarahamse (Laser Research Center) Dr Nicolette Houreld (Laser Research Center)

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