Speaker
Ms
Ratsimandresy Holinirina Dina Miora
(Laser Research Institute, Stellenbosch University)
Description
A fluorescence microscopy is an essential tool in the life sciences. In particular, it can be used for tracking a single molecule in a living cell or organic substance such as a polymer. Single molecule trajectory analysis allows an efficient method to explore intermolecular dynamics. Introduction of a technique named double helix point spread function enables accurate localization and orientation determination of a single fluorescent molecule in three dimensions [1]. It consists of changing the phase of the electric field of the emitter in order to make the intensity pattern sensitive to position and orientation [2]. The method uses optical Fourier processing through a 4f imaging system and a modelled phase loaded onto a spatial light modulator placed on the Fourier plane of the 4f imaging pathway [3]. The intensity pattern is changed from the conventional point spread function to a double helix point spread function. In this work, we introduce the phase mask in order to get a double helix point spread function then show results for its usefulness in fluorescence microscopy.
References
[1] Pavani SR, Thompson MA, Biteen JS, Lord SJ, Liu N, Twieg RJ, Piestun R, Moerner WE. Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function. Proceedings of the National Academy of Sciences. 2009 Mar 3;106(9):2995-9.
[2] Roider C, Jesacher A, Bernet S, Ritsch-Marte M. Axial super-localisation using rotating point spread functions shaped by polarisation-dependent phase modulation. Optics express. 2014 Feb 24;22(4):4029-37.
[3] Backer AS, Moerner WE. Extending single-molecule microscopy using optical Fourier processing. The Journal of Physical Chemistry B. 2014 May 12;118(28):8313-29.
Level for award<br> (Hons, MSc, <br> PhD, N/A)?
MSc
Apply to be<br> considered for a student <br> award (Yes / No)?
Yes
Would you like to <br> submit a short paper <br> for the Conference <br> Proceedings (Yes / No)?
No
Main supervisor (name and email)<br>and his / her institution
Dr Gurthwin W Bosman (gwb@sun.ac.za)
Department of Physics, Laser Research Institute, Stellenbosch University, South Africa
Prof Erich G Rohwer (egr@sun.ac.za)
Department of Physics, Laser Research Institute, Stellenbosch University, South Africa
Primary author
Ms
Ratsimandresy Holinirina Dina Miora
(Laser Research Institute, Stellenbosch University)
