22-26 March 2021
Zoom Conference
Africa/Johannesburg timezone
Biophysics in and for Africa

Theoretical comparison of real-time single-particle tracking techniques

22 Mar 2021, 15:00
20m
Zoom Conference

Zoom Conference

Virtual Event
Oral Presentation Molecular biophysics Molecular biophysics

Speaker

Bertus van Heerden (University of Pretoria)

Description

One of the main challenges in studying single biomolecules in a native or near-native environment is their constant diffusion. An approach to solving this problem is real-time single particle tracking (SPT). In this study, we used statistical calculations and dynamic simulations to compare the orbital, Knight’s Tour and MINFLUX localization methods, in the context of fluorescence-based and interferometric scattering (iSCAT) approaches. While the Knight’s Tour method can track the fastest diffusion, MINFLUX achieves the greatest precision. The relative success of iSCAT vs fluorescence is strongly dependent on the particle size, and the photophysical properties and density of the fluorophores.

Primary author

Bertus van Heerden (University of Pretoria)

Co-author

Tjaart Krüger (University of Pretoria)

Presentation Materials

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