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SUMMARY:Theoretical comparison of real-time single-particle tracking techn
 iques
DTSTART;VALUE=DATE-TIME:20210322T130000Z
DTEND;VALUE=DATE-TIME:20210322T132000Z
DTSTAMP;VALUE=DATE-TIME:20260714T154450Z
UID:indico-contribution-6920@events.saip.org.za
DESCRIPTION:Speakers: Bertus van Heerden (University of Pretoria)\nOne of 
 the main challenges in studying single biomolecules in a native or near-na
 tive environment is their constant diffusion. An approach to solving this 
 problem is real-time single particle tracking (SPT). In this study\, we us
 ed statistical calculations and dynamic simulations to compare the orbital
 \, Knight’s Tour and MINFLUX localization methods\, in the context of fl
 uorescence-based and interferometric scattering (iSCAT) approaches. While 
 the Knight’s Tour method can track the fastest diffusion\, MINFLUX achie
 ves the greatest precision. The relative success of iSCAT vs fluorescence 
 is strongly dependent on the particle size\, and the photophysical propert
 ies and density of the fluorophores.\n\nhttps://events.saip.org.za/event/2
 13/contributions/6920/
LOCATION:Zoom Conference
URL:https://events.saip.org.za/event/213/contributions/6920/
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