Speaker
Description
Breast cancer is a form of cancer that affects women and is regarded as the second leading cause of cancer-related deaths. Phyto photodynamic therapy is a promising therapeutic approach adopted in cancer research, which uses plant-derived bioactive compounds in combination with photosensitizers to induce apoptosis in cancer cells. Dicoma anomala is an African medicinal plant, that is widely used in the treatment of various diseases. In this research, D. anomala extracts were used in combination with zinc phthalocyanine tetrasulphonic acid (ZnPcS4) to induce cell death in MCF-7 breast cancer cells. The cells were treated with different concentrations (25, 50 and 100 µg/mL) of methanolic root extract and the dose response results were used to calculate the IC50 value.(85 µg/mL). Morphological changes were observed using inverted microscope. The lactate dehydrogenase (LDH) cytotoxicity and ATP proliferation assays were performed to determine the cytotoxic effect of the extract and ZnPcS4 after 24 h of treatment. The morphological results showed a significant decrease in cell population while LDH level was increased and ATP levels were decreased in dying cells.. The outcome of this research suggests the potential medicinal benefits of D. anomala and ZnPcS4 in breast cancer treatment.
Apply to be considered for a student ; award (Yes / No)?
Yes
Level for award;(Hons, MSc, PhD, N/A)?
MSc
