12-15 July 2011
Saint George Hotel
Africa/Johannesburg timezone

Effect of Low Intensity Laser Irradiation (LILI) and Epidermal Growth Factor (EGF) on Adult Human Adipose Derived Stem Cells (hADSCs)

14 Jul 2011, 11:30


Oral Presentation Track F - Applied and Industrial Physics Applied


Mr Bernard Mvula (Doctoral Student)


The study investigated the effects of low intensity laser radiation (LILI) and epidermal growth factor (EGF) on adult adipose derived stem cells (hADSCs) isolated from human adipose tissue. Isolated cells were cultured in complete Dulbeco’s Modified Eagle Medium (DMEM) containing 10% featal bovine serum (FBS), EGF and antibiotics and incubated at 37°C in 85% humidified atmosphere of 5% CO2. Semi-confluent monolayers of ADSCs were exposed to low intensity laser at 5 J/cm2 using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0, 24 and 48 h post irradiation. Application of low intensity laser irradiation and EGF on human ADSCs at 5 J/cm2 increased the viability and proliferation of these cells. Therefore low intensity laser therapy would probably enhance differentiation and proliferation of stem cells in vitro and these cells could be used in reconstructive surgery and tissue engineering.

Would you like to <br> submit a short paper <br> for the Conference <br> Proceedings (Yes / No)? Yes
Level (Hons, MSc, <br> &nbsp; PhD, other)? PhD
Consider for a student <br> &nbsp; award (Yes / No)? Yes

Primary author

Mr Bernard Mvula (Doctoral Student)


Prof. Heidi Abrahamse (SupervisorI)

Presentation Materials