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SUMMARY:Effect of Low Intensity Laser Irradiation (LILI) and Epidermal Gro
 wth Factor (EGF) on Adult Human Adipose Derived Stem Cells (hADSCs)
DTSTART;VALUE=DATE-TIME:20110714T093000Z
DTEND;VALUE=DATE-TIME:20110714T094500Z
DTSTAMP;VALUE=DATE-TIME:20260911T112218Z
UID:indico-contribution-5257@events.saip.org.za
DESCRIPTION:Speakers: Bernard Mvula (Doctoral Student)\nThe study investig
 ated the effects of low intensity laser radiation (LILI) and epidermal gro
 wth factor (EGF) on adult adipose derived stem cells (hADSCs) isolated fro
 m human adipose tissue.  Isolated cells were cultured in complete Dulbeco
 ’s Modified Eagle Medium (DMEM) containing 10% featal bovine serum (FBS)
 \, EGF and antibiotics and incubated at 37°C in 85% humidified atmosphere
  of 5% CO2.  Semi-confluent monolayers of ADSCs were exposed to low intens
 ity laser at 5 J/cm2 using 636 nm diode laser.  Cell viability and prolife
 ration were monitored using adenosine triphosphate (ATP) luminescence and 
 optical density at 0\, 24 and 48 h post irradiation. Application of low in
 tensity laser irradiation and EGF on human ADSCs at 5 J/cm2 increased the 
 viability and proliferation of these cells.  Therefore low intensity laser
  therapy would probably enhance differentiation and proliferation of stem 
 cells in vitro and these cells could be used in reconstructive surgery and
  tissue engineering.\n\nhttps://events.saip.org.za/event/7/contributions/5
 257/
LOCATION: Acro3
URL:https://events.saip.org.za/event/7/contributions/5257/
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