14-18 November 2022
Africa/Johannesburg timezone
Big Science and Big Goals for Africa

In vivo crystallization of protein targets for African Sleeping Sickness (Trypanosoma brucei) Therapeutics

15 Nov 2022, 16:45
30m
AfLS Plenary

Speaker

Prof. Lars Redeke (Universität zu Lübeck Institut für Biochemie)

Description

Spontaneous protein crystallization within living cells has been observed several times in nature, e.g. for storage proteins in seeds. In vivo crystal growth can also occur during gene over-expression, as particularly discovered in baculovirus-infected insect cells [1]. We have recently shown that these in vivo crystals represent valuable targets for structural biology after isolation from the cell. Applying serial crystallography techniques at an X-ray free-electron laser (XFEL) as well as using a highly brilliant synchrotron source, single crystal diffraction pattern were collected and combined to yield high-resolution structural information of the associated fully glycosylated protein

Primary author

Prof. Lars Redeke (Universität zu Lübeck Institut für Biochemie)

Presentation Materials

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