4-8 July 2016
Kramer Law building
Africa/Johannesburg timezone
Paper Review: Initial screening in progress
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Photobiomodulation of Isolated Lung Cancer Stem cells

Presented by Ms. Anine CROUS on 6 Jul 2016 from 11:30 to 11:50
Type: Oral Presentation
Session: Photonics
Track: Track C - Photonics

Abstract

Background: Research has uncovered that one of the plausible reasons for cancer relapse is the existence of stem like cells, possessing cancer properties, called cancer stem cells (CSCs). Cancer research is highly focused on improving current cancer treatments. One method of targeted cancer therapy is Photodynamic therapy (PDT), where Low Intensity Laser Irradiation (LILI), along with a photochemical compound, is used. When implementing a mechanism by which CSCs are targeted, LILI might pose as a viable treatment option. Studies have shown that using high fluences of LILI (HF-LILI) can induce cell death in normal and neoplastic cells. Further investigations on cell death induced by HF-LILI of CSCs still needs to be explored. Methodology: Lung CSCs were isolated using the stem cell marker CD 133 and were exposed to LILI at wavelengths of 636, 825 and 1060 nm at fluences ranging from 5 J/cm2 to 40 J/cm2. Post irradiation biochemical assays were conducted to monitor cellular responses including; proliferation and cytotoxicity, after 24 hours incubation. Discussion: Studies have indicated that LILI, when treating lung CSCs, can induce either a bio-stimulatory or bio-inhibitory effect depending on the wavelength and fluence used. This study indicated successful cell damage of lung CSCs when using HF-LILI, as well as, stimulation of ATP production, when using lower fluences of LILI.

Award

Yes

Level

MSc

Supervisor

Prof Heidi Abrahamse, habrahamse@uj.ac.za, University of Johannesburg

Paper

Yes

Permission

Yes

Place

Location: Kramer Law building
Address: UCT Middle Campus Cape Town
Room: 5C


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