Mathematical models of whole-body dynamics have advanced our understanding of human integrative systems that regulate physiological processes such as metabolism, temperature, and blood pressure. For most of these whole-body models, baseline parameters describe a 35-year-old young adult man who weighs 70 kg. As such, even among adults those models may not accurately represent half of the...
Recent advancements in super-resolution microscopy have enabled unprecedented insights into the spatial organization of cellular structures. In this talk, I will present a series of methodological innovations that synergistically integrate fluorescence-lifetime single-molecule localization microscopy (FL-SMLM) [1,2], image scanning microscopy (ISM) [3,4], and metal-/graphene-induced energy...
Water, in its many states, has a pivotal role in biology. But resolving it at the molecular level has been a challenge. Here, we resolve and describe how water determines the way in which an external stimulus, light in this example, intimately controls how the stimulus is conformational changes in membrane receptors in response to a stimulus, and capturing their functionally relevant dynamics,...
Understanding biological function at the molecular level requires direct visualization of macromolecular structure. For decades, structural biology has relied on approaches such as X ray crystallography and nuclear magnetic resonance spectroscopy. Over the past decade, cryo electron microscopy has rapidly matured into a central method for protein structure determination, expanding the scope of...
The current model of mitotic spindle assembly proposes that microtubules nucleate at spindle poles and grow inward to capture chromosomes. However, recent structural studies reveal that spindles are composed of short microtubules that do not span the full pole-to-chromosome distance. It remains unclear how short, disconnected microtubules collectively generate and transmit the forces necessary...
