Fluorescence (spontaneous emission) is a highly sensitive probe for a multitude of molecular processes during the light-dependent steps of photosynthesis in numerous organisms. In living organisms, the fluorescence signal is dwarfed by reflection and scattering; however, the signal-to-noise ratio can be significantly enhanced by gating the fluorescence to sub-msshort excitation pulses through...
F.O. Brenya1 and N.N. Houreld1
1Laser Research Centre, University of Johannesburg, Johannesburg, South Africa
E-mail: nhoureld@uj.ac.za
Antimicrobial photobiomodulation (aPBM) with blue light (400-490 nm) is a promising non-invasive adjunct therapy for infected chronic diabetic foot ulcers (DFUs). Still, its fluence-dependent effects on human dermal fibroblasts remain poorly defined....
The generation of functional insulin-producing beta (β)-cells from stem cells represented a promising therapeutic strategy for diabetes mellitus. Photobiomodulation (PBM), a non-invasive light-based approach, has emerged as a potential regulator of cellular metabolism, proliferation, and differentiation through mitochondrial stimulation and bioenergetic modulation. This study investigated the...
Multimodal optical microscopy has recently converged with artificial intelligence (AI) to the computational prediction of fluorescence‑based molecular
contrast from label‑free measurements. Fluorescence plays a crucial role in linking microscopy and spectroscopy at molecular level, enabling image formation from the cellular to the molecular detail. A challenging development in this field is...
The real-time tracking of single particles is a vastly under-developed experimental technique but it offers great potential in understanding molecular dynamics. A suitable tracking system comprises three key components, viz., a position sensor, a control system, and an output actuator. The position sensor enables accurate prediction of the particle location. For this component, various...
Photobiomodulation (PBM) refers to the application of low-level light at specific wavelengths delivered either continuously or in pulses with low and constant energy densities in order to modulate cellular activity. This study investigated the influence of PBM at 525 nm (green), 825 nm (near-infrared; NIR) and a combination wavelength of both on adipose-derived stem cell (ADSC) using assays...
