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SUMMARY:Optimizing Photobiomodulation For Smooth Muscle Differentiation of
  Adipose-Derived Stem Cells Using Retinoic Acid and TGFβ in a Two-Dimensi
 onal Model
DTSTART;VALUE=DATE-TIME:20260325T144000Z
DTEND;VALUE=DATE-TIME:20260325T150000Z
DTSTAMP;VALUE=DATE-TIME:20260426T030119Z
UID:indico-contribution-10346@events.saip.org.za
DESCRIPTION:Speakers: Christevie Mbuyu (Laser Research Centre)\nPhotobiomo
 dulation (PBM) refers to the application of low-level light at specific wa
 velengths delivered either continuously or in pulses with low and constant
  energy densities in order to modulate cellular activity. This study inves
 tigated the influence of PBM at 525 nm (green)\, 825 nm (near-infrared\; N
 IR) and a combination wavelength of both on adipose-derived stem cell (ADS
 C) using assays which assess cellular parameters such as proliferation\, v
 iability\, mitochondrial function\, extracellular matrix (ECM) production\
 , migration and differentiation towards a smooth muscle cell (SMC)   in th
 e presence of growth factors like retinoic acid (RA) and transforming grow
 th factor-β (TGF-β). ADSCs were exposed to fluences of 5 J/cm² and 10 J
 /cm² and assessed using metabolic (ATP)\, cytotoxicity (LDH)\, Giemsa for
  morphology\; mitochondrial membrane potential (MMP)\, collagen quantifica
 tion\, migration analysis and immunofluorescence staining of stemness and 
 SMC associated markers. Morphological analysis demonstrated that ADSCs ini
 tially maintained a typical spindle-shaped  morphology across all experime
 ntal groups. However\, fluence-dependent PBM effects became evident over t
 ime. Cultures exposed to 5 J/cm² maintained higher cell density and proli
 feration\, whereas 10 J/cm² groups showed sparser cultures and reduced gr
 owth\, consistent with the biphasic Arndt-Schulz response. ATP analysis su
 pported this trend with lower fluence groups demonstrating enhanced metabo
 lic activity by day 7\, while higher fluence groups showed reduced prolife
 ration. LDH levels remained low across all PBM-treated groups\, confirming
  the absence of cytotoxic effects. Mitochondrial analysis indicated a gene
 rally stable MMP values during early stages with increased levels at later
  stages suggesting mitochondrial adaptation during differentiation. Collag
 en production showed an early increase followed by a decline at later time
  points\, suggestive of ECM remodelling during cellular maturation. Migrat
 ion analysis revealed differences in cell motility between treatment group
 s\, indicating functional variations associated with PBM exposure. Immunof
 luorescence confirmed the progressive expression of smooth muscle associat
 ed markers including smooth muscle α actin\, desmin\, calponin and SMMHC 
 during the experimental period. Collectively\, these findings demonstrate 
 that PBM modulates ADSC behaviour in a fluence-dependent fashion and may s
 upport smooth muscle lineage development under inductive culture condition
 s further highlighting the potential role of PBM in regenerative medicine 
 applications.\n\nhttps://events.saip.org.za/event/272/contributions/10346/
LOCATION:
URL:https://events.saip.org.za/event/272/contributions/10346/
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