BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:Fluorescence-Lifetime Super-Resolution Microscopy DTSTART;VALUE=DATE-TIME:20260326T090000Z DTEND;VALUE=DATE-TIME:20260326T094000Z DTSTAMP;VALUE=DATE-TIME:20260426T115550Z UID:indico-contribution-10330@events.saip.org.za DESCRIPTION:Speakers: Jörg Enderlein (University of Göttingen)\nRecent a dvancements in super-resolution microscopy have enabled unprecedented insi ghts into the spatial organization of cellular structures. In this talk\, I will present a series of methodological innovations that synergistically integrate fluorescence-lifetime single-molecule localization microscopy ( FL-SMLM) [1\,2]\, image scanning microscopy (ISM) [3\,4]\, and metal-/gr aphene-induced energy transfer (MIET/GIET) imaging [5–7]. These approac hes collectively offer isotropic three-dimensional resolution at the nanom eter scale\, multiplexed imaging capabilities\, and robustness against chr omatic aberrations.\n\nFirst\, I will discuss our work on MIET and GIET mi croscopy\, which exploit distance-dependent quenching phenomena near metal lic or graphene interfaces to determine the axial position of single emitt ers with sub-10 nm accuracy. The combination of MIET with dSTORM or DNA-PA INT provides truly isotropic 3D resolution\, extending the reach of locali zation microscopy into the axial dimension without interferometric complex ity.\n\nSecond\, I will highlight the development of fluorescence lifetime DNA-PAINT (FL-PAINT)\, a technique that enables multi-target super-resolu tion imaging through fluorescence lifetime multiplexing without fluid exch ange. By utilizing orthogonally designed imager strands conjugated to fluo rophores with distinct lifetimes\, we achieve simultaneous imaging of mult iple targets in the dense intracellular environment.\n\nLastly\, I will in troduce our latest development of fluorescence-lifetime image scanning mic roscopy SMLM (FL-iSMLM)\, which achieves a near twofold enhancement in lat eral resolution by integrating a single-photon detector array into a confo cal laser scanning microscope. This method combines the localization preci sion of ISM with the multiplexing power of fluorescence-lifetime detection \, enabling sub-5 nm resolution in fixed cells while simultaneously allowi ng discrimination of targets based solely on their fluorescence lifetimes. \n\nReferences\n[1] J. C. Thiele\, D. A. Helmerich\, N. Oleksiievets\, R. Tsukanov\, E. Butkevich\, M. Sauer\, O. Nevskyi\, and J. Enderlein\, Confo cal Fluorescence-Lifetime Single-Molecule Localization Microscopy\, ACS Na no 14\, 14190 (2020).\n[2] J. C. Thiele\, O. Nevskyi\, D. A. Helmerich\, M . Sauer\, and J. Enderlein\, Advanced Data Analysis for Fluorescence-Lifet ime Single-Molecule Localization Microscopy\, Front. Bioinform. 1\, 740281 (2021).\n[3] C. B. Müller and J. Enderlein\, Image Scanning Microscopy\, Phys. Rev. Lett. 104\, 198101 (2010).\n[4] N. Radmacher\, O. Nevskyi\, J. I. Gallea\, J. C. Thiele\, I. Gregor\, S. O. Rizzoli\, and J. Enderlein\, Doubling the resolution of fluorescence-lifetime single-molecule localiza tion microscopy with image scanning microscopy\, Nat. Photon. 18\, 1059 (2 024).\n[5] A. I. Chizhik\, J. Rother\, I. Gregor\, A. Janshoff\, and J. En derlein\, Metal-induced energy transfer for live cell nanoscopy\, Nature P hoton 8\, 124 (2014).\n[6] A. Ghosh\, A. Sharma\, A. I. Chizhik\, S. Isban er\, D. Ruhlandt\, R. Tsukanov\, I. Gregor\, N. Karedla\, and J. Enderlein \, Graphene-based metal-induced energy transfer for sub-nanometre optical localization\, Nat. Photonics 13\, 860 (2019).\n[7] J. C. Thiele\, M. Jung blut\, D. A. Helmerich\, R. Tsukanov\, A. Chizhik\, A. I. Chizhik\, M. Sch nermann\, M. Sauer\, O. Nevskyi\, and J. Enderlein\, Isotropic Three-Dimen sional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Tr ansfer\, Science Advances 8\, 14190 (2021).\n\nhttps://events.saip.org.za/ event/272/contributions/10330/ LOCATION: URL:https://events.saip.org.za/event/272/contributions/10330/ END:VEVENT END:VCALENDAR