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SUMMARY:The efficacy of PAM fluorometry as a tool to quantify heat stress 
 in wheat
DTSTART;VALUE=DATE-TIME:20260325T130000Z
DTEND;VALUE=DATE-TIME:20260325T132000Z
DTSTAMP;VALUE=DATE-TIME:20260426T115549Z
UID:indico-contribution-10320@events.saip.org.za
DESCRIPTION:Speakers: Sarah Burnett (Univeristy of Pretoria)\nFluorescence
  (spontaneous emission) is a highly sensitive probe for a multitude of mol
 ecular processes during the light-dependent steps of photosynthesis in num
 erous organisms.  In living organisms\, the fluorescence signal is dwarfed
  by reflection and scattering\; however\, the signal-to-noise ratio can be
  significantly enhanced by gating the fluorescence to sub-msshort excitati
 on pulses through a non-invasive technique known as pulse-amplitude-modula
 ted (PAM) fluorometry. Wheat is an economically important crop that is sus
 ceptible to heat stress and consequent yield reduction at temperatures abo
 ve 30°C. However\, the changes to molecular processes that cause the decr
 ease in yield have not been well reported.  In this study\, PAM fluorometr
 y was used to investigate the effects of high temperatures on the energy t
 ransfer pathways during the light-dependent steps of photosynthesis. The q
 uantum efficiency of energy conversion from light to chemical energy was n
 ot significantly altered at 30°C but was reduced by 10.7% at 35°C. We sh
 ow that the biological changes due to the heat shock response to heat stre
 ss can be measured at a time resolution of 30 seconds.  PAM fluorometry\, 
 and thus fluorescence\, is able to provide information about the effects o
 f heat stress on electron transport during light-dependent photosynthesis.
  This opens many possible directions of study\, such as investigating the 
 effects of different types of stress on photosynthesis or further modellin
 g the photosynthetic energy-transfer pathways under heat stress.\n\nhttps:
 //events.saip.org.za/event/272/contributions/10320/
LOCATION:
URL:https://events.saip.org.za/event/272/contributions/10320/
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