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SUMMARY:Laser-Based Photobiomodulation Enhances Beta Cell Differentiation 
 and Functional Insulin Production in Immortalised Adipose-Derived Stem Cel
 ls
DTSTART;VALUE=DATE-TIME:20260325T134000Z
DTEND;VALUE=DATE-TIME:20260325T140000Z
DTSTAMP;VALUE=DATE-TIME:20260426T030125Z
UID:indico-contribution-10316@events.saip.org.za
DESCRIPTION:Speakers: Olukemi Daramola (University of Johannesburg)\nThe g
 eneration of functional insulin-producing beta (β)-cells from stem cells 
 represented a promising therapeutic strategy for diabetes mellitus. Photob
 iomodulation (PBM)\, a non-invasive light-based approach\, has emerged as 
 a potential regulator of cellular metabolism\, proliferation\, and differe
 ntiation through mitochondrial stimulation and bioenergetic modulation. Th
 is study investigated the effect of laser-based PBM on the differentiation
  of immortalised adipose-derived stem cells (ADSCs) into functional β-cel
 ls under both two-dimensional (2D) and three-dimensional (3D) culture cond
 itions.\nImmortalised ADSCs were exposed to defined laser parameters to ev
 aluate their capacity to enhance β-cell lineage commitment and functional
  insulin production. Cellular health\, viability\, and metabolic activity 
 were assessed using multiple complementary assays. Intracellular adenosine
  triphosphate (ATP) quantification determined mitochondrial bioenergetic a
 ctivity\, while lactate dehydrogenase (LDH) release was measured to evalua
 te cytotoxicity and membrane integrity. Morphological changes associated w
 ith differentiation were examined using Giemsa staining\, and β-cell-spec
 ific insulin granule formation was confirmed using dithizone (DTZ) stainin
 g. Additionally\, Live/Dead assays were performed to assess overall cell v
 iability and survival within both 2D monolayer and 3D scaffold-based cultu
 re systems.\nIt was hypothesized that laser-based PBM enhanced mitochondri
 al activity\, improved cellular viability\, and promoted the generation of
  metabolically active\, insulin-producing β-cells\, with enhanced outcome
 s observed in 3D culture systems due to improved cell–cell and cell–ma
 trix interactions.\nThis study aimed to advance understanding of laser-bas
 ed PBM mechanisms in stem cell differentiation and to support the developm
 ent of non-invasive strategies for β-cell engineering and regenerative di
 abetes therapies.\n\nhttps://events.saip.org.za/event/272/contributions/10
 316/
LOCATION:
URL:https://events.saip.org.za/event/272/contributions/10316/
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