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SUMMARY:Green-Synthesized Silver Nanoparticle–Liposomal ZnPcS4 Nanoplatf
 orm for Enhanced Photodynamic Therapy in Breast Cancer
DTSTART;VALUE=DATE-TIME:20260325T080000Z
DTEND;VALUE=DATE-TIME:20260325T082000Z
DTSTAMP;VALUE=DATE-TIME:20260427T052858Z
UID:indico-contribution-10310@events.saip.org.za
DESCRIPTION:Speakers: Alexander Chota (University of Johannesburg\, Laser 
 Research Centre)\nBreast cancer remains a formidable challenge in oncology
  despite significant advancements in treatment modalities. Conventional th
 erapies such as surgery\, chemotherapy\, radiation therapy\, and hormonal 
 therapy have been the mainstay in managing breast cancer for decades. Howe
 ver\, a subset of patient’s experiences treatment failure\, leading to d
 isease recurrence and progression. Therefore\, this study investigates the
  therapeutic potential of green-synthesized silver nanoparticles (AgNPs) u
 sing an African medicinal plant (Dicoma anomala methanol root extract) as 
 a reducing agent for combating breast cancer. AgNPs were synthesized using
  the bottom-up approach and later modified with liposomes (Lip) loaded wit
 h photosensitizer (PS) zinc phthalocyanine tetra-sulfonate (Lip@ZnPcS4) us
 ing thin film hydration method. The successful formation and Lip modificat
 ion of AgNPs\, alongside ZnPcS4\, were confirmed through various analytica
 l techniques including UV–Vis spectroscopy\, Fourier-transform infrared 
 spectroscopy (FT-IR)\, high-resolution transmission electron microscopy (H
 R-TEM)\, scanning electron microscopy (SEM) and energy dispersive X-ray sp
 ectroscopy (EDS). Following a 24 h treatment period\, MCF-7 cells were ass
 essed for viability using 3-[4\,5-dimethylthiazol-2-yl]-2\,5 diphenyl tetr
 azolium bromide (MTT viability assay)\, cell death analysis using mitochon
 drial membrane potential (MMP) (ΔΨm)\, Annexin V-fluorescein isothiocyan
 ate (FITC)-propidium iodide (PI) kit\, and caspase- 3\, 8 and 9 activities
 . The experiments were repeated four times (n = 4)\, and the results were 
 analyzed using SPSS statistical software version 27\, with a confidence in
 terval set at 0.95. The synthesized nanoparticles and nanocomplex\, includ
 ing AgNPs\, AgNPs-Lip\, Lip@ZnPcS4\, and AgNPs-Lip@ZnPcS4\, exhibited nota
 ble cytotoxicity and therapeutic efficacy against MCF-7 breast cancer cell
 s. Notably\, the induction of apoptosis\, governed by the upregulation of 
 apoptotic proteins i.e.\, caspase 8 and 9 activities. In addition\, caspas
 e 3 was not expressed by MCF-7 cells in both control and experimental grou
 ps. Given the challenging prognosis associated with breast cancer\, the fi
 ndings underscore the promise of liposomal nanoformulations in cancer phot
 odynamic therapy (PDT)\, thus warranting further exploration in clinical s
 ettings*emphasized text*\n\nhttps://events.saip.org.za/event/272/contribut
 ions/10310/
LOCATION:
URL:https://events.saip.org.za/event/272/contributions/10310/
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