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SUMMARY:Fluorinated Surfactants for Membrane-Protein Extraction
DTSTART;VALUE=DATE-TIME:20210322T150000Z
DTEND;VALUE=DATE-TIME:20210322T152000Z
DTSTAMP;VALUE=DATE-TIME:20260719T220958Z
UID:indico-contribution-6960@events.saip.org.za
DESCRIPTION:Speakers: Kenechi Kanayo Onyia (University of Nigeria\, Nsukka
 )\nSurfactants carrying either fully or partially fluorinated alkyl chains
  are conventionally thought to be poor solubilisers of lipids and membrane
  proteins because of their lipophobicity. New fluorinated surfactants of d
 ifferent headgroups have been developed. We show that these compounds coul
 d substitute detergents' function without much interference with membrane 
 proteins' functionality. Their self-assembly and solubilising properties w
 ere studied by the use of isothermal titration calorimetry (ITC)\, dynamic
  light scattering (DLS)\, and gel electrophoresis (SDS-PAGE). Micellisatio
 n was found to be mainly driven by entropy\, and the critical micellar con
 centration (CMC) decreased with increasing hydrocarbon chain length. Notab
 ly\, some of these surfactants solubilise lipid vesicles at room temperatu
 re and extract important membrane proteins directly from *Escherichia coli
 * membranes. Our findings demonstrated promising\, mild detergent activity
  for maltose-based fluorinated surfactants in membrane-protein extraction 
 and applications compared to the lactose-based compounds.\n\nhttps://event
 s.saip.org.za/event/213/contributions/6960/
LOCATION:Zoom Conference
URL:https://events.saip.org.za/event/213/contributions/6960/
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