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SUMMARY:Effects of photodynamic therapy on A375 Melanoma cells using alumi
 nium phthalocyanine photosensitizer
DTSTART;VALUE=DATE-TIME:20210726T101500Z
DTEND;VALUE=DATE-TIME:20210726T103000Z
DTSTAMP;VALUE=DATE-TIME:20260915T053746Z
UID:indico-contribution-7193@events.saip.org.za
DESCRIPTION:Speakers: Bridgette Mkhobongo (Laser Research Centre)\nMetasta
 tic Melanoma (MM) is highly aggressive and is among cancers causing major 
 global deaths annually. It is imperative to find therapies that can elimin
 ate MM and has become a major concern due to the potential for cancer rela
 pse and metastasis\, as well as the disease being accounted to be resistan
 t to multiple forms of therapy. This *in vitro* study explores the effect 
 of Photodynamic Therapy (PDT) using an Aluminium Phthalocyanine Photosensi
 tizer (AlPcS4Cl) at 673 nm and a fluency of 5 J/cm2\, in targeting Melanom
 a cells (A375). Dose dependent response of AlPcS4Cl was studied on both A3
 75 and fibroblast (WS1) cell lines and the IC50 calculated from this. Sign
 ificant post-irradiation signs of cell death were detected using microscop
 y and biochemical assays. Cell viability testing showed increased damaged 
 cells taking up Trypan Blue Dye. A decrease in cell proliferation was obse
 rved through the measurement of Adenosine Triphosphate (ATP) content. An i
 ncreased release of Lactate Dehydrogenase (LDH) content due to cytotoxicit
 y with increasing doses of AlPcS4Cl was measured. The study suggested an e
 ffective treatment against Melanoma cells. Enhanced capabilities of PDT fo
 r MM could possibly be achieved through gold nanoparticle (AuNP) activated
  increased uptake of AlPcS4Cl photosensitizer\, targeting their quiescent 
 cancer stem cells.\n\nhttps://events.saip.org.za/event/206/contributions/7
 193/
LOCATION:North-West University Potchefstroom Campus
URL:https://events.saip.org.za/event/206/contributions/7193/
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