from 28 June 2015 to 3 July 2015 (Africa/Johannesburg)
Africa/Johannesburg timezone
SAIP2015 Proceeding published on 17 July 2016
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The Fundamentals of Single Molecule Microscopy.

Presented by Dr. Gurthwin BOSMAN on 1 Jul 2015 from 11:10 to 11:30
Type: Oral Presentation
Session: Photonics
Track: Track C - Photonics

Abstract

In the last decade, single molecule microscopy has become the technique of choice when sub-diffraction imaging is required [1]. Sub-diffraction imaging using optical microscopes requires that single molecule fluorescent markers be imaged stochastically (in techniques like stochastic optical reconstruction microscopy (STORM) or photoactivated localization microscopy (PALM)) or with very low concentrations such that only a small fraction of the emitters are imaged at any given time [2]. When combining the abovementioned techniques with point-spread function tailoring, three dimensional localization accuracies well below 50 nm are readily achieved [3]. In this presentation the fundamental requirements for achieving high localization accuracies with single molecule microscopy will be discussed. References: [1] B. Flier, et. al. Heterogeneous Diffusion in Thin Polymer-Films as observed by High-Temperature Single Molecule Fluorescence Microscopy; J. Am. Chem. Soc. 2012, 134, 480-488. [2] C. Galbraith, et al. Super-resolution microscopy at a glance; J Cell Sci. 2011, 124(10): 1607–1611. [3] S. Pavani, et. al. Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function; PNAS 2009, 106(9), 2995-2999.

Award

No

Level

N/A

Supervisor

Gurthwin Bosman, gwb@sun.ac.za, SU

Paper

No

Permission

Yes

Primary authors

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