SAIP 2011 Support
Effect of Low Intensity Laser Irradiation (LILI) and Epidermal Growth Factor (EGF) on Adult Human Adipose Derived Stem Cells (hADSCs)
Presented by Mr. Bernard MVULA on 14 Jul 2011 from 11:30 to 11:45
Type: Oral Presentation
Track: Track F - Applied and Industrial Physics
<p>The study investigated the effects of low intensity laser radiation (LILI) and epidermal growth factor (EGF) on adult adipose derived stem cells (hADSCs) isolated from human adipose tissue. Isolated cells were cultured in complete Dulbeco’s Modified Eagle Medium (DMEM) containing 10% featal bovine serum (FBS), EGF and antibiotics and incubated at 37°C in 85% humidified atmosphere of 5% CO2. Semi-confluent monolayers of ADSCs were exposed to low intensity laser at 5 J/cm2 using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0, 24 and 48 h post irradiation. Application of low intensity laser irradiation and EGF on human ADSCs at 5 J/cm2 increased the viability and proliferation of these cells. Therefore low intensity laser therapy would probably enhance differentiation and proliferation of stem cells in vitro and these cells could be used in reconstructive surgery and tissue engineering.
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